| * | King Horn HS Facility Records | 1:33.09 | Jason Holloway, Eric Blair, Darrius Thomas, Nicholas Lane | Wayne | 2024 |
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Kings | A | Santiago | Magrino | Johnson | Felix | 1:36.32 |
| 2 | Miami East | A | 1:37.82 | ||||
| 3 | Lima Perry | A | Mcway | Williams | McFadden | Glenn | 1:39.67 |
| 4 | Marion Local | A | 1:39.85 | ||||
| 5 | Kings | B | Christensen | Modesto | Stuart | Modesto | 1:40.00 |
| 6 | Liberty Center | A | Lawniczak | Kahle | Rohrs | Rosebrook | 1:40.94 |
| 7 | Spencerville | A | 1:41.15 | ||||
| 8 | Kings | C | Hertlein | Sutherland | Thompson | Hurst Jr | 1:42.00 |
| 9 | Indian Lake | A | Cosby | Holbrook | Clary | Lacher | 1:43.00 |
| 10 | Marion Local | B | 1:43.00 | ||||
| 11 | Lima Senior | A | Maxwell | Webster | Law | Horton | 1:44.27 |
| 12 | Tinora | A | Jimenez | Gentit | Smith | Hoffman | 1:44.94 |
| 13 | Kalida | A | Fortman | Kuhlman | Zeller | Meyer | 1:45.52 |
| 14 | Lebanon | C | 1:46.49 | ||||
| 15 | Lebanon | B | 1:46.49 | ||||
| 16 | Maumee Va. C.D. | A | Skinner | Kiehl | Wang | Wheeler | 1:47.20 |
| 17 | New Bremen | A | Bernhold | Whitlatch | Gerasimchik | Baker | 1:48.97 |
| 18 | Arcanum | A | Arbogast | Hittle | Hootman | Pfahler | 1:50.32 |
| 19 | Hughes | A | Al-Lateef | Cephas | Robinson | Duncan | 1:53.39 |
| 20 | New Bremen | B | Gerasimchik | Lefeld | Niekamp | Timmerman | 2:00.00 |
| 21 | Dixie | A | Gentry | Hardin | Jackle | McNew | NT |