| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | A | Schield | Bailey | Lyle | Valdivia | DNS | |
| 3 | Juniata |
C | Smith | Downs | Quinn | McGowan | DNS |
| 4 | Juniata |
A | Massare | Thomas | Fritzges | Ndikwe | 3:27.29 |
| 5 | Juniata |
B | Wilson | Alexander | Robb | Kelly | 3:35.00 |
| 6 | Potomac State College | A | Timbrook | Sharp | Sealey | Lawrence | DNS |
| 7 | Penn State T&F Club | A | Christou | Cohen | Hu | Parks | 4:40.00 |