| PL | JD | # | Athlete | Team | Time | HPL |
|---|---|---|---|---|---|---|
| 630 | SCOTT, Breslin | Trojan Track | 34.18 | |||
| 366 | BARNES, Elijah | Hershey Blaze | 35.41 | |||
| 632 | STOKES, Keith | Trojan Track | 36.33 | 3 (1) | ||
| 559 | KING, Jace | Nittany Track | 37.99 | |||
| 386 | TANNER, Sean | Hershey Blaze | 38.45 | |||
| 404 | JACKSON, Trayion | Homewood Bulldawgs | 39.03 | |||
| 376 | MCLAUGHLIN, Charlie | Hershey Blaze | 39.29 | |||
| 556 | JAMES, Elijah | Nittany Track | 39.57 | 3 (2) | ||
| 305 | HOUSTON-GARDNER, Messiah | Ballistic Response | 39.84 | |||
| 387 | WILLIAMS JR, Matthew | Hershey Blaze | 39.99 | 2 (1) | ||
| 448 | CUNNINGHAM, Graydon | Juniata Valley Striders | 40.45 | 2 (2) | ||
| 414 | WILLIAMS, Muhammed | Homewood Bulldawgs | 42.70 | 3 (3) | ||
| 382 | OSEGHALE, Samuel | Hershey Blaze | 43.71 | 3 (4) | ||
| 575 | RAPTOSH, Maddox | Nittany Track | 46.50 | |||
| 401 | FOWLER, Nafeze | Homewood Bulldawgs | 46.81 | 2 (3) | ||
| 624 | GRAY II, Sheldon | Trojan Track | 47.11 | 2 (4) | ||
| 464 | HINES, Alex | Mountain Top Track & Field | 47.35 | 3 (5) | ||
| 555 | HERBICK, Roman | Nittany Track | 1:00.56 | 2 (5) | ||
| 653 | DOUGHERTY, Miles | Unattached | 1:18.88 | 2 (6) |