| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1 | 151 | KALANDARIA, Luka | SO | State College 06 | 1:00.14 |
| 2 | 194 | YARNELL, Adin | SR | State College 06 | 54.15 |
| 3 | 28 | GERITANO, Jackson | FR | Central Dauphin 03 | 1:02.24 |
| 4 | 164 | MORNINGSTAR, Matt | SR | State College 06 | 53.51 |
| 5 | 25 | COTTON, Kaden | SO | Central Dauphin 03 | 1:00.24 |
| 6 | 150 | JOLIN, David | SO | State College 06 | 54.06 |
| 7 | 36 | NUSS, Ezra | FR | Central Dauphin 03 | 1:02.24 |
| 8 | 142 | HAMMOND, Lex | JR | State College 06 | 1:00.00 |