| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 121 | MARTIN, Josie | Unattached | NT |
| 3 | 30 | EDOR, Serena | Nittany Track and Field | NT |
| 4 | 115 | MACFARLANE, Carina | Unattached | 38.00 |
| 5 | 80 | REID, Isabella | Nittany Track and Field | 40.80 |
| 6 | 117 | MANEY, Adeline | Unattached | NT |
| 7 | 128 | ROWLAND, Pennsylvania | Unattached | NT |