| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 307 | HUNTER, aleeyah | Capital Area Forerunners | 36.34 |
| 3 | 290 | HICKS, Simone | Hershey Blaze | 34.76 |
| 4 | 51 | GREGORY, Paige | YMCA Of Indiana County | 33.00 |
| 5 | 249 | WYLAND, Ember | Nittany Track and Field | 33.86 |
| 6 | 284 | ELBY, Aliya | Hershey Blaze | 36.29 |
| 7 | 27 | SHUNIAK, Julia | Mountain Top Track & Field | 38.15 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 170 | MADAUS, Peyton | Nittany Track and Field | NT |
| 3 | 315 | CAMPBELL, Maylee | PA Flight Club | NT |
| 4 | 312 | ROWLAND-HARRIS, Sage | Capital Area Forerunners | 39.95 |
| 5 | 277 | BURKS, Kennedi | Hershey Blaze | NT |
| 6 | 40 | KEISTER, Kylie | Trojan Track | NT |
| 7 | 296 | MCDONALD, Taylor | Hershey Blaze | NT |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 239 | VAZQUEZ DANJO, Aina | Nittany Track and Field | NT |
| 3 | 220 | SHORTER, Raya | Nittany Track and Field | NT |
| 4 | 188 | ORZOLEK, Leiana | Nittany Track and Field | NT |
| 5 | 215 | SCHARF, Genevieve | Nittany Track and Field | NT |
| 6 | 225 | STAGER, Noel | Nittany Track and Field | NT |