| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 308 | KING, Zahra | Capital Area Forerunners | 55.04 |
| 3 | 311 | PURDY, Eyslyn | Capital Area Forerunners | 45.12 |
| 4 | 276 | ABROKWA, Aubrey | Hershey Blaze | 37.96 |
| 5 | 19 | ENDLER, Charlotte | Mountain Top Track & Field | 40.84 |
| 6 | 264 | KENNER, Emory | Unattached | 52.20 |
| 7 | 87 | CHAMBERLAIN, Kaye | Nittany Track and Field | NT |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 223 | STAGER, Nanette | Nittany Track and Field | NT |
| 3 | 297 | RESSLER, Aria | Hershey Blaze | NT |
| 4 | 41 | LAUBER, Emmett | Trojan Track | NT |
| 5 | 164 | LENKEY, Clarisse | Nittany Track and Field | NT |
| 6 | 300 | RESSLER, Journei | Hershey Blaze | NT |
| 7 | 29 | TAKACS, Indie | Mountain Top Track & Field | NT |