| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 301 | BAKER, James | Capital Area Forerunners | 25.70 |
| 2 | 165 | LIADIS, Tristan | Nittany Track and Field | 24.75 |
| 3 | 101 | DONMOYER, Elliott | Nittany Track and Field | 23.81 |
| 4 | 243 | WEIS, Jake | Nittany Track and Field | 23.06 |
| 5 | 282 | DEFREHN, Grant | Hershey Blaze | 23.09 |
| 6 | 293 | KOLLIE, Makai | Hershey Blaze | 24.67 |
| 7 | 62 | SPALLONE, Bryson | River Valley Track Club | 25.55 |
| 8 | 143 | HORTON, Henry | Nittany Track and Field | 29.05 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 271 | SIEGEL, Alex | Unattached | NT |
| 2 | 156 | KENNEDY, Christian | Nittany Track and Field | NT |
| 3 | 141 | HOLT, Kameron | Nittany Track and Field | NT |
| 4 | 316 | CAMPBELL, Victor | PA Flight Club | NT |
| 5 | 94 | CROTEAU, Oliver | Nittany Track and Field | NT |
| 6 | 149 | JOLIN, David | Nittany Track and Field | NT |
| 7 | 59 | MILLER, Samuel | River Valley Track Club | NT |
| 8 | 33 | WECHTENHISER, Chance | Mountain Top Track & Field | NT |