| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 31 | TROSKY, Tiffany | Mountain Top Track & Field | 32.84 |
| 3 | 113 | ELIAS MS., Eleanor | Nittany Track and Field | 27.78 |
| 4 | 318 | OTT, Zoe | PA Flight Club | 26.67 |
| 5 | 274 | WELMON, Vivian | Unattached | 27.19 |
| 6 | 304 | EDRINGTON, Euriah | Capital Area Forerunners | 30.76 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 3 | 278 | CHOW, Sydney | Hershey Blaze | NT |
| 4 | 43 | MCALLISTER, Carmen | Trojan Track | NT |
| 5 | 150 | JOLIN, Estella | Nittany Track and Field | NT |
| 6 | 217 | SEKHON, Skyler | Nittany Track and Field | NT |