| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 34 | BARR, Charlotte | Trojan Track | 30.16 |
| 2 | 175 | MCCANTS, Melina | Nittany Track and Field | 28.65 |
| 3 | 104 | DUKEMAN, Alayna | Nittany Track and Field | 28.33 |
| 4 | 266 | MAPLES, Bridget | Unattached | 27.40 |
| 5 | 42 | LAUGHMAN, Leah | Trojan Track | 27.90 |
| 6 | 261 | HOEY, Addyson | Unattached | 28.59 |
| 7 | 100 | DIXON, Hannah | Nittany Track and Field | 29.10 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 54 | PAYNE, Aaliyah | YMCA Of Indiana County | 36.01 |
| 3 | 291 | HUGHES, Ella | Hershey Blaze | 32.44 |
| 4 | 16 | DRENNING, Emma | Mountain Top Track & Field | 30.22 |
| 5 | 15 | DEMERITT, Isabell | Mountain Top Track & Field | 31.66 |
| 6 | 176 | MCCONNELL, Summer | Nittany Track and Field | 33.38 |
| 7 | 90 | CLAAR, Madeline | Nittany Track and Field | NT |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 2 | 24 | PIOTTI, Mya | Mountain Top Track & Field | NT |
| 3 | 179 | MENSAH, Edna | Nittany Track and Field | NT |
| 4 | 283 | DURHAM, Jue'Lyssa | Hershey Blaze | NT |
| 5 | 147 | JACKSON, Matilda | Nittany Track and Field | NT |
| 6 | 310 | NASH, Kalea | Capital Area Forerunners | NT |
| 7 | 238 | VARCOE, Eva | Nittany Track and Field | NT |