| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 14 | CARNEY, Ashlyn | Mountain Top Track & Field | 32.80 |
| 2 | 198 | RAISH, Elsa | Nittany Track and Field | 31.97 |
| 3 | 190 | PARETTE, Quinn | Nittany Track and Field | 30.54 |
| 4 | 305 | HADLEY, Brooklynn | Capital Area Forerunners | 29.70 |
| 5 | 28 | STORM, Olivia | Mountain Top Track & Field | 29.98 |
| 6 | 185 | OKAFOR, Chidera | Nittany Track and Field | 30.60 |
| 7 | 20 | ENDLER, Vivian | Mountain Top Track & Field | 32.32 |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 36 | CATTALO, Giada | Trojan Track | NT |
| 2 | 95 | CROTEAU, Samantha | Nittany Track and Field | 36.85 |
| 3 | 50 | GREGORY, Blaire | YMCA Of Indiana County | 34.00 |
| 4 | 268 | MAPLES, Samantha | Unattached | 32.92 |
| 5 | 263 | KENNER, Corbin | Unattached | 32.94 |
| 6 | 159 | KOCH, Emmalene | Nittany Track and Field | 36.77 |
| 7 | 76 | BAYSINGER, Kimberly | Nittany Track and Field | NT |
| 8 | 337 | NASH, Kahlia | Capital Area Forerunners | NT |
| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 44 | SHRADER, Mackenzie | Trojan Track | NT |
| 2 | 256 | JONES, Kayden | Climax | NT |
| 3 | 128 | GORE, Meghan | Nittany Track and Field | NT |
| 4 | 92 | COULTER, Sawyer | Nittany Track and Field | NT |
| 5 | 106 | DUNAHAY, Skylar | Nittany Track and Field | NT |
| 6 | 130 | HAAS, Sabine | Nittany Track and Field | NT |
| 7 | 178 | MCWHIRTER, Sarah | Nittany Track and Field | NT |