| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 86 | BURRELL, Asher | Nittany Track and Field | 4:40.75 |
| 2 | 324 | STUBE, Jonathan | Unattached | 5:10.00 |
| 3 | 331 | DEICHERT, Marisa | Unattached | 5:20.00 |
| 4 | 333 | WALKER, Susie | Unattached | 5:55.00 |
| 5 | 330 | URBAN, Eli | Unattached | 6:00.00 |
| 6 | 328 | PETERSEN, Alexa | Unattached | NT |
| 7 | 322 | HIPP, Andrew | Unattached | 6:30.00 |
| 9 | 323 | ROSS, Davis | Unattached | 6:30.00 |
| 10 | 332 | BURRELL, Aaron | Unattached | 9:00.00 |
| 11 | 329 | SAVITSKI, Steve | Unattached | 9:30.00 |