| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lucy Addison Middle School | A | NT | |
| 2 | James Breckinridge Middle School | A | NT | |
| 3 | Shawsville Middle School | A | 5:24.51 | Blacksburg Bruin Quad Meet (MS) |
| 4 | Fort Chiswell Middle School | A | 6:05.26 | Abingdon ENT Associates MS Opene |
| 5 | Galax | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Maury River Middle School | B | DNS | Henley MS Dual/Tri/Quad |
| 2 | Pulaski County Middle School | A | 5:09.00 | Wallace Middle School Invitation |
| 3 | Christiansburg Middle School | A | 4:51.20 | Wallace Middle School Invitation |
| 4 | Auburn Middle School | A | 4:56.93 | Giles Middle School Meet #1 |
| 5 | George Wythe (Wytheville) | A | 5:10.00 | |
| 6 | Maury River Middle School | A | 5:16.48 | Blacksburg Bruin Quad Meet (MS) |