| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Patrick Henry (G. Spring) | A | Ferguson | Tuggle | Thompson | Croson | DNS | Unverified: |
| 3 | Lee | A | Penix | Carter | Grace | Chadwell | DNS | Verified |
| 4 | University School of Johnson Cit | A | Williams | Buda | Mayhew | Papas | 3:59.00 | Unverified: Dobyns-Bennett HS March 16th |
| 5 | Jefferson County High School | A | Mills | Satterfield | Christian | Lovell | DNS | Verified |
| 6 | John S. Battle | A | DNS | Verified | ||||
| 7 | Tennessee High School | A | Childress | Romans | Hutson | Harris | 4:05.24 | Verified |
| 8 | University School of Johnson Cit | B | Shaw | Mussard | Scheller | Osborne | DNS | Unverified: Dobyns-Bennett HS March 16th |
| 9 | Gate City High School | A | Gardner | Dean | Hamilton | Krantz | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jefferson County High School | B | Giles | Jones | Annis | Jackson | DNS | Verified |
| 2 | Elizabethton High School | A | Stephens | Lucas | Siepert | Norris | DNS | Verified |
| 3 | West Ridge High School | A | Steedman | Wolfe | Ricketts | Hawkins | 3:44.69 | Verified |
| 4 | Blacksburg High School | A | Hager | Foley | Bandera | Roach | 3:31.00 | Unverified: |
| 5 | Science Hill High School | A | Jones | Deakins | Edmisten | Horne | 3:30.00 | Verified |
| 6 | Science Hill High School | B | Mitchell | Cline | Guhn | Lockman | 3:30.00 | Verified |
| 7 | Daniel Boone High School | A | Battel | Cline | Goodall | Lewis | 3:42.28 | Verified |
| 8 | Blacksburg High School | B | Wilson | Bagchi | Whitfield | Strahm | 3:45.00 | Unverified: |
| 9 | Abingdon High School | A | Pillion II | Bundy | Molinary | Duke | 3:50.00 | Unverified: |