| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Rye Cove | A | Barnette | Goins | Bowen | Sloan | 53.60 | Verified |
| 4 | Eastside High School | A | Mullins | Mullins | Adkins | Ward | 49.74 | Verified |
| 5 | John I. Burton | A | Ball | Culbertson | Stidham | Coffey | 53.07 | Verified |
| 6 | Wise Central High School | A | Sexton | Gilliam | Partin | Freeman | DQ | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Thomas Walker | A | Lowe | McCurry | Norris | Kimberlin | DNS | |
| 2 | Twin Springs | A | Kilgore | Gibbs | Horne | Glymp | 47.17 | Verified |
| 4 | Abingdon High School | A | 44.42 | Verified | ||||
| 5 | Union High School | A | Cochran | McCray | Welch | Welch | 44.87 | Verified |
| 6 | Ridgeview High School | A | Baker | Collins | Counts | Beavers | 46.77 | Unverified: Lee High All Comers Meet |
| 7 | Castlewood | A | 47.28 | Verified | ||||
| 8 | Honaker | A | Musick | Bandy | Lowe | Musick | 49.10 | Verified |