| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Providence Academy | A | 48.13 | Verified |
| 2 | Volunteer High School | A | DNS | Verified |
| 3 | Daniel Boone High School | A | 47.14 | Verified |
| 4 | Blacksburg High School | A | 46.50 | Unverified: |
| 5 | Science Hill High School | A | 44.57 | Verified |
| 6 | West Ridge High School | A | 46.08 | Verified |
| 7 | Tennessee High School | A | 46.83 | Verified |
| 8 | Patrick Henry (G. Spring) | A | 47.21 | Unverified: Falcon Early Bird Spring Meet |
| 9 | Cherokee High School | A | DNS | Verified |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Blacksburg High School | B | NT | Unverified: |
| 3 | West Ridge High School | B | NT | Verified |
| 4 | Abingdon High School | A | NT | Unverified: |
| 5 | John S. Battle | A | 50.69 | Verified |
| 6 | University School of Johnson Cit | A | NT | Unverified: |
| 7 | David Crockett High School | A | DNS | Unverified: |
| 8 | Science Hill High School | B | NT | Verified |