| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1A | Katzenbach, Hannah | FR | Tonopah | NT | |
| 1B | Luckett, Alexandra | FR | Andre Agassi | NT | |
| 2A | Davy, Mady | SO | Calvary Chapel | 2:55.93 | |
| 2B | Hexamer, Chloe | SO | The Meadows | DNS | |
| 2C | Nathan, Svetlana | FR | Andre Agassi | NT | |
| 3A | Villanueva, Karina | SO | Beatty | 3:09.41 | |
| 3B | Lee, Ashley | SO | The Meadows | NT | |
| 4A | Utterback, Kaitlyn | FR | Tonopah | DNF | |
| 4B | Minton, Pashaun | JR | Andre Agassi | NT |