| # | DBRC | 49.64 | Daniel Greaves | Wheeling Jesuit |
| ! | Marietta College | 50.02 | Tom Moss | Marietta |
| Athlete | Team | Seed | |
|---|---|---|---|
| 1 | MULLENAX, DANIEL | Charleston (W.V.) | 49.50 |
| 2 | VANDUSEN, BRIAN | Wheeling University | 50.00 |
| 3 | IVEY, DAIMAR | Wheeling University | 50.35 |
| 4 | CAMPBELL, KOHL | Wheeling University | 50.35 |
| 5 | ROYSTER, TYHIR | Carlow | 50.80 |
| 6 | WHITE, DAMIEN | Glenville St. | 51.00 |
| 7 | WAUGH, DEREK | Glenville St. | 51.00 |
| 8 | OLIVAS, KEVIN | Charleston (W.V.) | 51.11 |
| 9 | SCARANGELLO, NICOLAS | Davis & Elkins | 51.59 |
| 10 | GLOVER, ROBERT | Glenville St. | 52.50 |
| 11 | DUDGEON, ANTHONY | Glenville St. | 52.50 |
| 12 | HARRISON, BRADLEY | Ohio Valley | 52.66 |
| 13 | WALKER, TYLER | Marietta | 52.70 |
| 14 | STROEBEL, ETHAN | Carlow | 53.10 |
| 15 | KELLEY, MARCUS | Glenville St. | 53.50 |
| 16 | BERMAN, CHRISTOPHER | Unattached | 54.00 |
| 17 | KELLEY, MARSHAWN | Glenville St. | 54.50 |
| 18 | KACOR, JARED | Charleston (W.V.) | 55.00 |
| 19 | MCCOMAS, MATTHEW | Charleston (W.V.) | 55.39 |
| 20 | BEISEL, JASON | Unattached | 56.00 |
| 21 | BROYLES, SHELDON | Muskingum | 56.68 |
| 22 | WITHROW, DAVID | Charleston (W.V.) | 1:05.11 |
| 23 | MAXWELL, PARKER | Salem (W.V.) | NT |
| 24 | DENNIS, CULLEN | Marietta | NT |
| 25 | LOVEGROVE, MARK | Ohio Valley | NT |