| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Muskingum | A | Lowry, Sydney | Neville, Spencer | Taylor, Kimberly | Double, Mary | DNS |
| 4 | Duquesne | A | Valeriano, Amberly | Evankovich, Bethany | Holmberg, Gabby | Beck, Chyna | 47.20 |
| 5 | Geneva | A | Younger, Olivia | McChesney, Judith | Swartzbaugh, Lydia | Ofalt, Lydia | 51.25 |