| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | South Pointe | A | Brown | Whitlock | Anderson | Davis | NT |
| 3 | Catawba Ridge HS | A | NT | ||||
| 4 | Nation Ford | A | Davis | Smith | Davis | Lucas | 48.50 |
| 5 | Rock Hill | A | McCrea | Rockeymore-Crews | Jones | Brown | 49.35 |
| 6 | Northwestern | A | Jones | Boyd | Ervin | Stewart | NT |