| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Lexington HS | A | Alexander | Gepfert | Davenport | Lewis | 3:48.46 |
| 3 | White Knoll | A | Banks | Johnson | Herriott-White | Peebles | 3:37.38 |
| 4 | River Bluff | A | Hart | Faison | Cook | Williams | 3:34.10 |
| 5 | Dutch Fork | A | Montgomery | Beard | Ross-El | Brown | 3:34.46 |
| 6 | Chapin | A | Terry | Dedmond | Chapman | Pieters | 3:41.41 |