| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Lancaster | A | Grier | Stover | Twitty | Curry | 43.78 |
| 2 | Emerald | A | Dean | Wright | Goodman | Key | 42.85 |
| 3 | Camden | A | Green | Loney | Smith | Boulware | 42.34 |
| 4 | South Pointe | A | Truesdale | Howze | Davis | Isley | 41.78 |
| 5 | AC Flora | A | Croffie | Betette | Gary | Offord | 42.15 |
| 6 | Fountain Inn High School | A | Curenton | Holliday | Flood | Tate | 42.52 |
| 7 | Richland Northeast | A | Willingham | Caesar | Gano | Smith | 42.98 |
| 8 | Dreher | A | Greene | Brown | Roach | Garrett | 43.96 |