| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Dreher | A | Roach | Cruz | Bolton | Garrett | 43.50 |
| 2 | Fountain Inn High School | A | Gray | Smith | Clay | Tate | 43.12 |
| 3 | Camden | A | Wesley | Wright | Boulware | Smith | 43.00 |
| 4 | South Pointe | A | Douglas | Watson | Howze | Visor | 42.73 |
| 5 | Richland Northeast | A | Newsome | Asuzu | Johnson | Smith | 42.92 |
| 6 | AC Flora | A | Croffie | Caudle | Mitchell | Kelson | 43.10 |
| 7 | Emerald | A | Dean | Goodman | Ryans | Key | 43.40 |