| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Caldwell Academy | A | Byrd | Sell | Byrd | Ortmann | NT | |
| 2 | Gaston Day | A | Thompson | La Rosa | Lichty | Brown | 58.10 | |
| 3 | Victory Christian Center | A | Johnson | Yearwood | Bowser | Boykins | DNF | |
| 4 | Burlington Christian Academy | A | Sweeney | Johnson | House | Mundy | 52.24 | |
| 5 | Fayetteville Academy | A | Edwards | Headley | Bowman | Domguia | 52.39 | |
| 6 | Hickory Christian Academy | A | Taylor | Bass | Lewis | Drum | 53.93 | |
| 7 | Wayne Country Day | A | Zitlalpopoca | Spicer | Bono | Pardo | 1:20.50 |