| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | O'Neal | A | Tracy | Stanton | Kennedy | Sartor | 49.29 | |
| 2 | Gaston Day | A | La | Fochtman | Manning | Pittman | 46.38 | |
| 3 | Caldwell Academy | A | Moles | Bates | Hubbard | Smith | 45.48 | |
| 4 | Fayetteville Academy | A | Haygood | Salisbury | Umerah | Al-Jurhanni | 44.00 | |
| 5 | Burlington Christian Academy | A | Godwin | Taylor | Vanhook | Stewart | 45.44 | |
| 6 | Victory Christian Center | A | Johnson | Whitlock | Coulter | Leak | 45.64 | |
| 7 | New Garden Friends | A | Sipp | Espinoza-Morales | Shamshoum | Price | 46.59 | |
| 8 | Wayne Country Day | A | Oguntade | Hodges | Jones | Chism | 52.24 |