| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Charlotte Latin | A | Paige | Karagosian | Luqmaan | Smith | NT | |
| 3 | Covenant Day | A | Dennison | Kocylowsky | Schmidt | Jameson | 1:41.65 | |
| 4 | Durham Academy | A | Black | Houser | Schmidt | Posada | 1:36.72 | |
| 5 | Wesleyan Christian Academy | A | Javier | Ngimbi Tandu | Javier | Pollio | 1:37.19 | |
| 6 | Cannon | A | Davis | Connell | Ravin | Sales | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Forsyth Country Day | A | Balliett-O'Neal | Rabadan Ribeiro | Horobey | Castro | 1:35.61 | |
| 2 | Charlotte Christian | A | Amasa | Amasa | Caswell | Caswell | 1:32.02 | |
| 3 | Rabun Gap-Nacoochee | A | Johnson | Willingham | Coleman | Salazar Delgado | 1:31.91 | |
| 4 | Christ School | A | Booker | Jones | Thompson | Murphy | 1:28.68 | |
| 5 | Providence Day | A | Sutton | Poku-Kankam | Barnes | Spencer | 1:30.85 | |
| 6 | Metrolina Christian Academy | A | Koos | Crenshaw | Little | McCormick | 1:31.99 | |
| 7 | Cary Academy | A | Oduor | Titcomb | Arhuidese | Lutz | 1:32.28 | |
| 8 | Charlotte Country Day | A | Hefner | Spruill | Dollard | Jewett | 1:36.50 |