| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Metrolina Christian Academy | A | Perez | Scisciani | Koos | Wolk | 51.67 | |
| 3 | Rabun Gap-Nacoochee | A | Zavala | Augustin | Shepard | Sinkovic | 51.17 | |
| 4 | Cannon | A | Gordon | Kenseth | Dobbins | Hall | 48.70 | |
| 5 | Durham Academy | A | Gunsch | Burrus | Savage | Lamb | 50.40 | |
| 6 | Providence Day | A | Gant | Gordon | Alli | Hee | 51.41 | |
| 7 | Forsyth Country Day | A | Tilley | Stewart | Gendrachi | Perrott | 53.34 | |
| 8 | Charlotte Country Day | A | Parsons | Little | Hill | Cromartie-Patterson | 54.43 |