| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wesleyan Christian Academy | A | Javier | Javier | Javier | White | 46.66 | |
| 3 | Forsyth Country Day | A | Gomez | Horobey | Pemberton | Castro | 45.87 | |
| 4 | Durham Academy | A | Dorsette | Dorsette | Jeffries | Black | DNS | |
| 5 | Ravenscroft | A | Vilsoee | Grant | Diggs | Del-Rosario | 44.96 | |
| 6 | Covenant Day | A | Barry | Schmidt | Dennison | Jameson | 46.07 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Charlotte Country Day | A | Hefner | Jewett | Wakefield | Dollard | 43.83 | |
| 2 | Christ School | A | Long-White | Jones | Booker | Murphy | 43.40 | |
| 3 | Cannon | A | Loggins | Ravin | Sales | Davis | 42.76 | |
| 4 | Charlotte Christian | A | Amasa | Amasa | Saylor | Frazier | 41.50 | |
| 5 | Rabun Gap-Nacoochee | A | Willingham | Dottin | Johnson | Willingham | 42.34 | |
| 6 | Providence Day | A | Sutton | Barnes | Poku-Kankam | Eich | 43.28 | |
| 7 | Charlotte Latin | A | Paige | Smith | Luqmaan | Karagosian | 43.66 | |
| 8 | Cary Academy | A | Arhuidese | Oduor | Dill | Lutz | 44.59 |