| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Trinity Academy of Raleigh | A | Cooper, Claire | Hepting, Madeleine | Woodfin, Ashley | Slaga, Lauren | 1:02.54 |
| 2 | Gaston Day | A | Visestamkul, Thanchanok | Moore, Melissa | Wilkinson, Melia | Jordan, Natalie | 1:00.24 |
| 3 | Grace Christian (Raleigh) | A | Monath, Lucy | Meeson, Kate | Gehringer, Beth | McGrath, Maren | 58.24 |
| 4 | St Davids | A | New, Karis | Wilson, Emily | Mears, Annie | Widin, Anna | 55.73 |
| 5 | Cary Christian | A | Carriker, Morgan Elizabeth | Chuchta, Sarah | Grubbs, Emerson | Deuser, Grace | 55.74 |
| 6 | Fayetteville Christian | A | Matthews, Hayley | Gardner, Diamari | Campbell, Victoria | Campbell, Majesty | 59.64 |
| 7 | Wayne Country Day | A | Ramsey, TT | Tabe, Beverly | Strickland, Katie | Williams, Destiny | 1:00.84 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | O'Neal | A | Bloom, Megan | Fox, Madison | Marsh, Tyra | Dailey, Lauren | 55.04 |
| 2 | Trinity Christian | A | Graham, Christa | Smith, Desiree | Harrells, Jaccara | Raynor-Brewington, Jada | DQ |
| 3 | Asheville Christian Academy | A | Hankley, Katie | Burnette, Hannah | Edwards, Maddie | Barker, Joy | 53.94 |
| 4 | Northside Christian | A | Mallette, Melonie | Jones, Zhateya | Washington, Dejah | Lockhart, Jonica | 53.84 |
| 5 | Burlington Christian Academy | A | Graves, Amanda | Donnelly, Mikaela | Workman, Victoria | Miller, Gracie | 53.84 |
| 6 | Victory Christian Center | A | Gaston, Terri | Mitchell, Assiyah | Stanton, Taniya | Wright, Christi | 54.34 |
| 7 | Woodlawn | A | Woodall, Amelia | Berini, Madison | Lolla, Carmen | Dailey, Megan | 54.77 |
| 8 | Caldwell Academy | A | Dodd, Natasha | Thompson, Cassie | Avis, Saige | Shields, Haverly | 55.05 |