| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | COMMUNITY CHRISTIAN | A | Huston, Andrew | Butler, Christian | Lassiter, Emmanuel | Achilles, Cameron | DNS | |
| 3 | GRACE CHRISTIAN (RALEIGH) | A | Burns, Russell | Goodwin, Wesley | Caldwell, Samuel | Carver, Josiah | 49.19 | |
| 4 | WESTCHESTER COUNTRY DAY | A | Webb, TJ | Neza, Terry | Tong, Hao | Weaver, Josh | 48.24 | |
| 5 | HICKORY CHRISTIAN ACADEMY | A | Speagle, Michael | McGrath, Tommy | Ruark, Keegan | Ruark, Keegan | 48.36 | |
| 6 | TRINITY ACADEMY OF RALEIGH | A | Wendt, William | Hevener, Thomas | Anderson, Ethan | Chaulk, Jordan | 50.41 | |
| 7 | ST DAVIDS | A | Batchelder, Robert | Brewer, Jonathan | Pipkin, Andrew | Walker, Houston | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | COASTAL CHRISTIAN | A | Murphy, Tommy | Dark, Marquise | Croom, Tafari | McEarlen, Connor | 47.45 | |
| 2 | VICTORY CHRISTIAN CENTER | A | Randolph, Makari | Coleman, Caleb | Dae, Marquis | Thompson, Tyreke | 46.82 | |
| 3 | FAYETTEVILLE CHRISTIAN | A | Sutton, Elias | Diggs, Derrick | Green, Jayvein | Jenkins, Jayden | 45.85 | |
| 4 | CFA ACADEMY | A | Treco, Nicholas | Crawford, D'Wayne | Gullatte, Jaylen | Jacobs, Bryan | 45.24 | |
| 5 | NORTHSIDE CHRISTIAN | A | Park, Chan | Taylor, Marco | Rennix, Malachi | McKoy, Michael | 45.56 | |
| 6 | BURLINGTON CHRISTIAN ACADEMY | A | Euliss, Michael | Jordan, Harrison | Wonganusom, Patrick | Hartley, Adam | 46.08 | |
| 7 | CARY CHRISTIAN | A | Dixon, Josh | Oentung, Timotius | Moore, Samuel | Armstrong, Chase | 47.30 | |
| 8 | CALDWELL ACADEMY | A | Browning, Chase | Kerr, Aspen | Quarandillo, Jansen | Peterman, Josh | 48.00 |