| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Cuthbertson | A | D'Errico | Taft | Anderson | Schihl | |
| 2 | Cuthbertson | B | Hinson | Mathers | Houp | Gaffney | |
| 3 | West Stanly | A | Preslar | Best | Shushlyakova | Burleson | |
| 4 | Piedmont | A | Tepera | Schie | Broadway | Tepera | |
| 5 | Union Academy | A | Leon | Moss | St. Clair | Isaacs | |
| 6 | Mooresville | A | Herrera | GenaoUrena | London | Newman | |
| 7 | Metrolina Christian Academy | A | Rutherford | Martin | Haser | Anderson |