| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 1 | Cuthbertson | A | Lightfoot | Dixon | Mcrorie | Weber | |
| 2 | Charlotte Latin | A | Guggenheimer | Khouri | Austin | DiSciullo | |
| 3 | Cuthbertson | B | Huertas | Ryan | Onthank | Heath | |
| 4 | Union Academy | A | Sanchez | Mills | Gonzales | Additon | |
| 5 | Charlotte Catholic | A | Patcha | Hay | Fitzpatrick | Traynum | |
| 6 | Mooresville | A | Byrd | Hensley | Cherry | Albaeck | |
| 7 | Parkwood | A | Franco-Gomez | Thomford | Maggio | Francis | |
| 8 | West Stanly | A | Preslar | Gannuscio | Grice | Jacobi | |
| 9 | Porter Ridge | A | Plotkin | Estwick | Santos | Daniels | |
| 10 | Metrolina Christian Academy | A | Koos | Rutherford | Fee | Capps |