| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Gardner-Webb |
A | Austin | Payne | Ivanov | Mitchell | 4:00.00 |
| 3 | Lincoln Memorial |
B | Close | Russell | Goins | Lewis | 3:59.77 |
| 4 | Ashland |
A | Perini | Below | Hampton | Woodruff | 3:45.67 |
| 5 | Marshall |
A | Santos | Murphy | Porter | King | 3:42.90 |
| 6 | Hillsdale |
A | Stamm | Eby | Dragovich | Behling | DNF |
| 7 | Lincoln Memorial |
A | Djokic | Mathis | Sells | Gibbs | 3:59.77 |
| 8 | Gardner-Webb |
B | Bour | Reece | Parandhamaia | Tretter | 4:12.00 |