| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Gardner-Webb |
B | Waller | Sastre | Morita | Bentley | 3:15.57 |
| 2 | Winthrop |
A | Johnson | Morgan | Washington | Vivanco | DNS |
| 3 | USC Upstate |
A | Thompson | Green | Leach | Watson | DNS |
| 4 | Ashland |
A | Roberts | Duncan | Phelps | Pospisil | 3:06.12 |
| 5 | Johnson C. Smith |
A | Towah | Jackson | Steele | DNS | |
| 6 | Hillsdale |
A | Meintjes | Brock | Calvert | Fagan | 3:14.89 |
| 7 | Gardner-Webb |
A | Hinton | Amour | Thornhill | Pittman | 3:15.57 |
| 8 | Charleston (W.V.) |
A | Burston | Mullenax | Smith | Sneed | DNS |