| # | Spartanburg County | 41.60 | Spartanburg | Spartanburg | 4/1/1990 |
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Byrnes | A | Byrd | Moncrieft | Cook | Weaver | 41.79 |
| 2 | Spartanburg | A | Tiswell | Harris | Rice | Miller | 41.86 |
| 3 | Dorman | A | Brannon | Foster | Foster | Bomar | 42.14 |
| 4 | Boiling Springs | A | Barrett | Eison | Thompson | Martin | 42.51 |
| 5 | Chesnee | A | Burguess | Webb | Franklin | Kesler | 43.63 |
| 6 | Broome | A | 43.66 | ||||
| 7 | Chapman | A | Pate | Thompson | Scott | Moorman | 44.08 |
| 8 | Woodruff | A | Johnson | Hiott | Tucker | Graybill | 44.22 |
| 9 | Landrum | A | Philbeck | Mashtare | Pien | Link | 45.53 |
| 10 | High Point Academy | A | 49.93 |