| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | Nation Ford | A | Davis | Smith | Davis | Lucas | 47.50 | Unverified: |
| 2 | Greer | A | Collins | Lewis | Clark-Lamille | Brantley | 49.65 | Verified |
| 3 | Byrnes | A | Wright | Rice | Griffin | Miller | 49.84 | Verified |
| 4 | Gaffney | A | Harris | Parker | Houze | Phillips | 50.16 | Verified |
| 5 | Boiling Springs | A | Grate | Garner | Dawkins | Watson | 50.87 | Unverified: |
| 6 | Dorman | A | Miller Mateen | Littlejohn | Boyce-Martin | Boozer | 51.05 | Verified |
| 7 | Northwestern | A | Ervin | Stewart | Bennett | Jackson | 51.15 | Verified |
| 8 | Broome | A | Garrett-Stevenson | Dimsdale | Humphries | Wilkins | 51.26 | Unverified: 3A-Upperstate 2022 |
| 9 | Spartanburg | A | Means-Boyd | Rice | Ferguson | Webster | 51.78 | Verified |
| 10 | Catawba Ridge HS | A | Hathaway | Gregory | Moore | Brailsford | 53.40 | Verified |
| 11 | Laurens | A | Brown | Cunningham | Grant | Montgomery | 54.57 | Verified |