| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Palm Beach Lakes |
A | DNS | |
| 4 | Martin County |
A | 44.95 | Jensen Beach Invitational 2026 |
| 5 | Dreyfoos |
A | DNS | FHSAA 2A District 8 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | A | 44.35 | FHSAA 3A Region 4 | |
| 2 | Suncoast |
A | 43.96 | Royal Palm Beach Invitational 2026 |
| 3 | Fort Pierce Central |
A | 43.75 | Cobra Relays 2025 |
| 4 | Dwyer |
A | 43.41 | FHSAA 4A Region 2 |
| 5 | Jupiter |
A | 43.56 | FHSAA 4A District 8 |
| 6 | Treasure Coast | A | 43.92 | SLC Championships 2025 |
| 7 | Sebastian River |
A | 44.15 | FHSAA 3A Region 4 |