| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Edward's |
A | Hebig | Callaghan | Cooper | Perrin | 10:28.68 | FHSAA 1A Region 4 |
| 2 | St. Edward's |
B | Hebig | Storts | Boulding | Ramsay | 10:28.68 | FHSAA 1A Region 4 |
| 3 | Vero Beach |
A | Watson | Johnson | Cooper | Lorenzo | 10:59.76 | Cobra Relays 2025 |
| 4 | Somerset College Prep | A | Jean-Noel | Guzman | Morales | Stoller | 11:41.76 | Pahokee Andre Powell Invitational |
| 5 | John Carroll |
A | Tokman | Russell | DUFFY | GUETTLER | 13:20.65 | Cobra Relays 2025 |