| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Tradition Prep | A | Derius | Phillippe | Parrel | Castro | 5:15.12 | Cobra Relays 2025 |
| 4 | St. Edward's |
A | Callaghan | Cannon | Storts | Perrin | 4:34.76 | MCHS Sarge Invitational 2025 |
| 5 | John Carroll |
A | Delince | VILLANUEVA | VAN RENS | GUETTLER | 4:45.47 | Jensen Beach Invite 2025 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Edward's |
B | Bischoff | Hall | Neimi | Shambo | 4:34.76 | MCHS Sarge Invitational 2025 |
| 2 | East River |
A | Winfield | Wilson | Ivy | Rodriguez | 4:14.28 | FHSAA 4A District 3 |
| 3 | Centennial |
A | Mathew | DiMarco | Garcia Guevara | Webber | 4:05.38 | FHSAA 4A Region 2 |
| 4 | Vero Beach |
A | Gibson | Wells | Domashinski | Overton | 3:50.70 | Florida Relays |
| 5 | Benjamin School |
A | Lewis | Balistreri | Downey | Travers | 4:01.60 | FHSAA 1A District 13 |
| 6 | Treasure Coast | A | Smith | Smith | Sama | Hatch | 4:08.63 | FHSAA 4A Region 2 |
| 7 | Fort Pierce Westwood |
A | Jackson | Holmes | Moody | Tolbert | 4:24.25 | Cobra Relays 2025 |