| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Benjamin School |
A | Balistreri | Lemanski | Travers | Himmich | 9:32.40 | FHSAA State Track & Field Championships (1A) |
| 2 | Treasure Coast | A | Wessel | Irizarry | Moore | Hatch | 10:07.83 | FHSAA 4A District 8 |
| 3 | St. Edward's |
A | Callaghan | Storts | Cooper | Perrin | 10:28.68 | FHSAA 1A Region 4 |
| 4 | East River |
A | Ivy | Falcon | Ojeda | Ojeda | 10:56.20 | FHSAA 4A District 3 |
| 5 | Vero Beach |
A | Morris | Converse | Johnson | Lorenzo | 10:59.76 | Cobra Relays 2025 |