| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Edward's |
A | Blackman | Donohoe | Wallace | Williams | 3:54.61 | Viera High School Invitational |
| 4 | Tradition Prep | A | Monaco | Beausejour-Chester | Salomon | Lopez | 3:49.72 | Jensen Beach Invitational 2026 |
| 5 | Tradition Prep | B | Chevres | Phillippe | Joseph | Quintavalle | 3:49.72 | Jensen Beach Invitational 2026 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vero Beach |
A | Chavis | Lewis | Cummings | Jacobs | 3:25.57 | FHSAA 4A Region 2 |
| 2 | Benjamin School |
A | Luiz | Etienne | Arwood | Gilles | 3:23.56 | Benjamin Invite #1 2025 (Sanctioned) |
| 3 | Treasure Coast | B | Forbes | Song | Ortado | Woodson | 3:19.87 | FHSAA 4A Region 2 |
| 4 | Centennial |
B | Ramezani | Salcedo | Jean-Baptiste | Raphael | 3:16.91 | FHSAA State Track & Field Championships (4A) |
| 5 | Centennial |
A | Benito | Thomas | Johnson | Bertrand | 3:16.91 | FHSAA State Track & Field Championships (4A) |
| 6 | Treasure Coast | A | Diejuste | Lemieux | Mathurin | Davis | 3:19.87 | FHSAA 4A Region 2 |
| 7 | East River |
A | Stafford | Quiredu | Mariot-Uribe | Rambless | 3:25.27 | Ocoee Relays |
| 8 | John Carroll |
A | BARBER | JACKSON | Pittman | Summerall | 3:39.36 | FHSAA 1A Region 4 |