| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Treasure Coast | A | Clark | Richardson | Reis | Issac | 3:44.88 | Cobra Relays 2023 |
| 3 | Centennial | A | Baez | Bacchas | Durbin | Miller | 3:37.24 | Jensen Beach Falcon Invitational |
| 4 | Fort Pierce Central | A | Beauford | Kaegi | Thompson | Domashinski | 3:28.21 | FHSAA 4A Region 3 |
| 5 | Fort Pierce Westwood | A | Wheeler | celisca | Scucess | Vincent | 3:36.72 | Treasure Lake Conference |
| 6 | Port St. Lucie | A | Desire | Alexis | Simms | Flores | 3:39.89 | FHSAA 3A District 13 |
| 7 | Tradition Prep | A | Dimarco | Joseph | Scayle | Drummond | 3:46.05 | Cobra Relays 2023 |