| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 636 | PARDO, NICOLE | JR | Boca Raton | NT | NT |
| 2 | 751 | MARSHALL, TAYLOR | JR | Martin County | 1:17.83 | MCHS Tiger Invitational 2023 |
| 3 | 148 | JONES, AKERAH | SR | Cardinal Gibbons | 1:07.86 | Louie Bing Invitational |
| 4 | 714 | SZPREGLEWSKI, JULIA | SO | Martin County | 1:16.38 | MCHS Tiger Invitational 2023 |
| 5 | 304 | GARCIA, ANA | JR | Archbishop McCarthy | 1:31.73 | Calvary Christian FtL Var Open 1 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 618 | BIJKER, VENICE | SO | Boca Raton | NT | NT |
| 3 | 189 | PORTER, ANIYA | SR | Fort Pierce Westwood | NT | NT |
| 4 | 598 | BANICA, GABRIELLA | JR | Boca Raton | NT | NT |
| 5 | 633 | KUPFERMAN, LILY | FR | Boca Raton | NT | NT |