| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wellington |
A | Maarsingh | Bentley | Carbocci | Lewis | 10:28.26 | 28th Annual Warrior Invitational 2024 |
| 2 | Archbishop McCarthy |
A | Coffin | Han | Ribeiro | Williams | 10:38.78 | FHSAA 3A District 15 |
| 3 | Boca Raton |
A | Perez | Granofsky | Pantelakis-Gunther | White | 10:42.09 | Royal Palm Beach Invitational |
| 4 | Dr. Joaquin Garcia | A | Brown | Gring | Meadows | Arce | 11:44.20 | Dr Garcia Invite #1 2025 |