| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Treasure Coast Classical | A | NT | |
| 4 | Melbourne Central Catholic |
A | 58.20 | Heritage Invitational 2 |
| 5 | Somerset College Prep | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Jensen Beach |
A | 55.86 | Jensen Beach Open 2024 |
| 2 | Martin County |
A | 52.37 | South Fork Meet #1 2024 |
| 3 | Fort Pierce Central |
A | 51.20 | Cobra Relays 2024 |
| 4 | Treasure Coast | A | 50.28 | 28th Annual Warrior Invitational 2024 |
| 5 | Palm Beach Gardens |
A | 51.08 | Palm Beach County Championships (Only PBC Schools) |
| 6 | King's Academy |
A | 52.06 | Kings Academy Invit 2 |
| 7 | South Fork |
A | 52.78 | Martin County Championships 2024 |
| 8 | St. Edward's |
A | 55.97 | South Fork Meet #1 2024 |