| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | GARDNER, Iris | King's Academy |
NT | NT |
| 4 | JEAN-LOUIS, Germene | Fort Pierce Central |
24.30 | SLC Championships 2024 |
| 5 | BENJAMIN, SANAA | Palm Beach Gardens |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BOND, Briana | Fort Pierce Central |
22.71 | Jupiter Invitational 2024 |
| 2 | FERGUSON, Mieca-Eeja | John Carroll |
21.33 | SLC Championships 2024 |
| 3 | NISULA, Tasha | King's Academy |
20.45 | Kings Academy Invit 2 |
| 4 | LEWIS, Tianna | Treasure Coast | 19.79 | 28th Annual Warrior Invitational 2024 |
| 5 | WALTERS, Kiara | Treasure Coast | 20.00 | 28th Annual Warrior Invitational 2024 |
| 6 | GEORGE, Cheyana | King's Academy |
20.49 | Spanish River Home Meet 2024 |
| 7 | EKSTROM, Tilde | Pine School |
22.10 | MCHS Tiger Invite 2024 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PETIT-HOMME, Jalah | Lake Worth Christian |
18.15 | Kings Academy Varsity Invit 1 |
| 2 | MILLER, Jahkya | Melbourne Central Catholic |
17.35 | Heritage Invitational 2 |
| 3 | BOURNE-MORGAN, Natasha | Somerset College Prep | 16.42 | TFRRS 03/01/24 - 16.00 |
| 4 | ODITA, Katherine | Palm Beach Gardens |
15.55 | Clyde Harris Invitational at Boynton |
| 5 | VEAL, Alana | Fort Pierce Central |
16.28 | Bill Wilson Invitational 2024 |
| 6 | SAMA, Arianna | Treasure Coast | 17.13 | Jensen Beach / Chandler Wealth Invitational 2024 |
| 7 | SZPREGLEWSKI, Julia | Martin County |
17.49 | Martin County Championships 2024 |
| 8 | WASH, Heather | South Fork |
18.22 | Martin County Championships 2024 |