| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Martin County |
A | 8:41.65 | MCHS Tiger Invite 2024 |
| 2 | Sebastian River |
A | DNS | Viera High School Track and Field Invitational |
| 3 | Moore Haven |
A | 9:20.07 | Antone Smith Muck City Invitational 2024 |
| 4 | Jensen Beach |
A | 9:24.76 | Jensen Beach Open 2024 |
| 5 | South Fork |
A | 10:00.30 | MCHS Tiger Relays |
| 6 | St. Edward's |
A | 10:58.59 | Benjamin Meet #1 2024 (FHSAA Sanctioned) |
| 7 | Pine School |
A | 11:43.17 | Jensen Beach Open 2024 |