| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Oxbridge Academy |
A | NT | |||||
| 2 | Tradition Prep | B | Russell | Lopez | Johnson | Riveron | NT | |
| 3 | South Fork |
A | Beard | Bonilla | Zeller | Mussenden | 47.73 | MCHS Fesh/Soph 2025 |
| 4 | Archbishop McCarthy |
A | Davis | Denson | Blanc | Grant | DNF | Pine Crest Panther Relays |
| 5 | Jupiter |
A | Jackson | Grashof | Sirmans | Madden | 43.87 | Benjamin Invite #1 2025 (Sanctioned) |
| 6 | Tradition Prep | A | Richardson | Atwell | Heredia | Drummond | NT | |
| 7 | Calvary Christian (PtStL) | A | Gonzalez | Alexis | Posten | Thompson | NT |