| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Roosevelt_Academy | A | DNS | |
| 3 | Hardee |
A | NT | |
| 4 | Frostproof |
A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | DeSoto County |
A | 2:00.30 | Sebring Relay Meet 2025 |
| 2 | Avon Park |
A | 1:56.70 | Sebring Relay Meet 2025 |
| 3 | Sebring |
A | 1:52.40 | Sebring Relay Meet 2025 |
| 4 | LaBelle |
A | DNS | Okeechobee Running Series 2025 |
| 5 | Okeechobee |
A | DQ | Sebring Relay Meet 2025 |
| 6 | Lake Placid |
A | NT |