| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Sebring |
A | 10:30.62 | FHSAA 3A Region 2 |
| 2 | LaBelle |
A | DNS | Charlotte Invitational |
| 3 | Hardee |
A | 11:25.27 | FHSAA 2A Region 3 |
| 4 | Frostproof |
A | 12:00.00 | |
| 5 | DeSoto County |
A | DNS | Immokalee Last Chance |
| 6 | Avon Park |
A | DNS | Avon Park Meet # 2 2025 |